Isolation of Phenolic Compound and Antioxidant from Saputangan Leaves (Maniltoa Grandiflora (A. Gray) Scheff)
Author(s):
Jhon Patar Sinurat , Universitas Sumatera Utara; Dr. Sovia Lenny, Universitas Sumatera Utara; Dr. FirmanSebayang, Universitas Sumatera Utara
Keywords:
Antioxidant, Isolation, Methyl Gallate, Phenolic Compound, Spectroscopy
Abstract:
Isolation & characterization of phenolic compound from Saputangan leaves (Maniltoa grandiflora (A. Gray) Scheff) and antioxidant activity have been carried out. The isolation process of phenolic compound was started through maceration process using methanol solvent, the partition using ethyl acetate and n-hexane, then the isolation using column chromatography. Characterization using UV-Vis, FT-IR, NMR and ESI-HRMS instruments. Antioxidant activity was carried out using DPPH method for phenolic compound. Based on the screening test, it can be stated that the Saputangan leaves contain phenolic compounds, flavonoids and terpenoids. Phenolic compound obtained has a weight of 32, 3 mg. The results of interpretation of spectroscopic data show that isolated phenolic compound is methyl gallate with the structure formula C8H8O5 and molecular weight of 184. Phenolic compound antioxidant as methyl gallate has IC50 values of 16,136 mg/L which can be categorized as very strong antioxidant.
Other Details:
| Manuscript Id | : | IJSTEV5I4016
|
| Published in | : | Volume : 5, Issue : 4
|
| Publication Date | : | 01/11/2018
|
| Page(s) | : | 60-65
|
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