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Isolation of Phenolic Compound and Antibacterial Activity of Phenolic Extract of Patah Tulang (Euphorbia Tirucalli. L)


Author(s):

Muhammad Furqan , Universitas Sumatera Utara; Sovia Lenny, Universitas Sumatera Utara; Lamek Marpaung, Universitas Sumatera Utara - Indonesia

Keywords:

E. Tirucalli, Screening, Antibacterial Activity, Phenolic Isolation, Spectroscopy

Abstract:

In this study, the phenolic compound was isolated and characterized, and their biological activity was assayed. The phenolic compound was isolated and characterized from the methanol extract using chromatographic techniques. The antimicrobial activity of the phenolic crude extract compound was assessed using disk diffusion method. Phytochemical investigation on the methanol crude extract of E. tirucalli resulted in the isolation of the phenolic compound structure approached to derivative of gallic acid. Their structures were deduced on the basis of UV-Vis Spectroscopy, FT-IR, 1H NMR, 13C NMR. The crude extract antimicrobial phenolic compound showed significant antibacterial activities against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Bacillus polymyxa in the disk diffusion assay. Inhibition zones were 9–15 mm. The maximum inhibition was shown at concentration 100 ppm against E. coli. The results showed that the isolated crude antimicrobial phenolic compound have significant antimicrobial activity, which can be used as antibiotics.


Other Details:

Manuscript Id :IJSTEV4I9038
Published in :Volume : 4, Issue : 9
Publication Date: 01/04/2018
Page(s): 145-150
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