Isolation of Phenolic Compounds of Patikan Kebo Leaves (Euphorbia Hirta. L)

Author(s):

Wisda Noviyanti Utami , Department of Mathematics and Natural Science, University of North Sumatera-Indonesia; Lamek Marpaung, Department of Mathematics and Natural Science, University of North Sumatera-Indonesia; Sovia Lenny, Department of Mathematics and Natural Science, University of North Sumatera-Indonesia

Keywords:

Euphorbia hirta. L, Phenolic Compounds, Isolation, Spectroscopy

Abstract:

Isolation and characterization of phenolic compound from methanol extract of Euphorbia hirta. L. has been conducted. The phenolic compound from methanol extracts isolated by several processes, such as TLC, column chromatography, and preparative TLC. The isolated phenolic compound is identified by UV-Vis spectrophotometer, FT-IR, 1H and 13C-NMR. The obtained phenolic compound is continued to the preparative TLC using chloroform: ethyl acetate (90:10, v/v) as eluent. The UV-Vis spectrum showed two peaks of maximum absorbance at 220 and 275 nm. Then, the FT-IR spectrum showed several peaks that confirmed the presence of functional group of derivative of phenolic compound, i.e. 3464, 1693, 1616, 1251, and 869 cm-1. 1H and 13C-NMR spectrum confirmed the isolated compound is derivative of phenolic compound, with data 3,81 (H-8, -OCH3) and 7,04 ppm (H-2, H-4 and H-6); 169 (-COO, C7), 146 (C3 and C5), 121 (C1), 110 (C2, C4, C6) and 52 ppm (-OCH3 C8), respectively. The E. hirta resulted in the isolation of the phenolic compound structure approached to derivative of gallic acid.

Other Details:

Manuscript Id	:	IJSTEV4I9047
Published in	:	Volume : 4, Issue : 9
Publication Date	:	01/04/2018
Page(s)	:	157-161

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