Enhancement and Detection of Chitinase Enzyme of Fungus for the Eradication of Pest

Author(s):

Priya. D , ADHIYAMAAN COLLEGE OF ENGINEERING; Sneka. S, ADHIYAMAAN COLLEGE OF ENGINEERING; Ramesh Babu. N. G., ADHIYAMAAN COLLEGE OF ENGINEERING; Karthikeyan. S, ADHIYAMAAN COLLEGE OF ENGINEERING

Keywords:

Chitinase, Chitosan, Pochonia Chlamydosporia, Beauveria Bassiana, Metarhizium Anisopliae, Trichoderma Harzianum

Abstract:

Chitinase is an enzyme having the ability to degrade chitin to a low molecular weight chitooligomers which have a wide range of applications. In the present study, chitinase was produced from the entomopathogenic fungi such as Pochonia chlamydosporia, Beauveria bassiana, Metarhizium anisopliae and Trichoderma harzianum using chitin as a carbon source. The enzyme was partially purified by ammonium sulphate precipitation and the activity was detected using colorimetric method. The enzyme showed the maximum activity in Trichoderma harzianum among the four fungal species at 40°C and pH 6.5. This enzyme could be used directly to control of pathogenic fungi and various pests in the egg stage itself.

Other Details:

Manuscript Id	:	IJSTEV3I2082
Published in	:	Volume : 3, Issue : 2
Publication Date	:	01/09/2016
Page(s)	:	169-172

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