CHARACTERISTICS OF CELLULASE PRODUCED BY Cellvibrio mixtus UV4 ISOLATED FROM SUGARCANE LEAF
Author(s):
Ummul Firmani , Faculty of Agriculture University of Muhammadiyah Gresik Gresik, East Java, Indonesia; Ni’matuzahroh, Faculty of Science and Technology University of Airlangga, Surabaya, Indonesia; Sri Sumarsih, Faculty of Science and Technology University of Airlangga, Surabaya, East Java, Indonesia
Keywords:
crude extract of cellulose, Cellvibrio mixtus UV4, specific activity of cellulose, sugarcane leaf
Abstract:
This study aims to characterize the crude extract of cellulase that produced by Cellvibrio mixtus UV4. This is an experimental research. Cellvibrio mixtus UV is obtained from the sugarcane leaf. Stages of this research were include (1) Sterilization of tools and materials; (2) production of cellulase by aerobic fermentation using liquid CMC 1% for 6 days; (3) isolation of crude extract cellulase; (4) determination of protein standard curve using Bovine Serum Albumin (BSA); (5) determination of the cellulase crude extract protein by the Bradford method; (6) determining the specific activity of crude extract cellulase; (8) analysis of the molecular weight of crude extract cellulase by SDS-PAGE. Analysis of the crude extract cellulase by SDS-PAGE produce proteins with molecular weights ranging from 127 kDa, 116 kDa, 98 kDa, 90 kDa, 70 kDa, 54 kDa and 46 kDa. Characteristics of the crude extract cellulase produced by C. mixtus UV4 resulting cellulase with activity of 0,011 U/mL, protein content 0.872 g/mL, and the specific activity of 12.62 U/mg.
Other Details:
| Manuscript Id | : | IJSTEV2I7004
|
| Published in | : | Volume : 2, Issue : 7
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| Publication Date | : | 01/02/2016
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| Page(s) | : | 249-251
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