IJSTE

CALL FOR PAPERS : Sep 2017

Submission Last Date
25-Sep--17
Submit Manuscript Online

FOR AUTHORS

FOR REVIEWERS

ARCHIEVES

DOWNLOADS

Open Access



CopyScape
Creative Commons License

CHARACTERISTICS OF CELLULASE PRODUCED BY Cellvibrio mixtus UV4 ISOLATED FROM SUGARCANE LEAF


Author(s):

Ummul Firmani , Faculty of Agriculture University of Muhammadiyah Gresik Gresik, East Java, Indonesia; Ni’matuzahroh, Faculty of Science and Technology University of Airlangga, Surabaya, Indonesia; Sri Sumarsih, Faculty of Science and Technology University of Airlangga, Surabaya, East Java, Indonesia

Keywords:

crude extract of cellulose, Cellvibrio mixtus UV4, specific activity of cellulose, sugarcane leaf

Abstract:

This study aims to characterize the crude extract of cellulase that produced by Cellvibrio mixtus UV4. This is an experimental research. Cellvibrio mixtus UV is obtained from the sugarcane leaf. Stages of this research were include (1) Sterilization of tools and materials; (2) production of cellulase by aerobic fermentation using liquid CMC 1% for 6 days; (3) isolation of crude extract cellulase; (4) determination of protein standard curve using Bovine Serum Albumin (BSA); (5) determination of the cellulase crude extract protein by the Bradford method; (6) determining the specific activity of crude extract cellulase; (8) analysis of the molecular weight of crude extract cellulase by SDS-PAGE. Analysis of the crude extract cellulase by SDS-PAGE produce proteins with molecular weights ranging from 127 kDa, 116 kDa, 98 kDa, 90 kDa, 70 kDa, 54 kDa and 46 kDa. Characteristics of the crude extract cellulase produced by C. mixtus UV4 resulting cellulase with activity of 0,011 U/mL, protein content 0.872 g/mL, and the specific activity of 12.62 U/mg.


Other Details:

Manuscript Id :IJSTEV2I7004
Published in :Volume : 2, Issue : 7
Publication Date: 01/02/2016
Page(s): 249-251
Download Article

IMPACT FACTOR

3.905

NEWS & UPDATES

Submit Article

Dear Authors, You can submit your article to our journal at the following link: http://www.ijste.org/Submit

Impact Factor

The Impact Factor of our Journal is 4.753 (Year - 2016)
3.905 (Year - 2015) 2.895(Year -2014)

Click Here

Submit Payment Online

Dear Authors, Now you can submit the payment receipt to our journal online at the following link: index.php?p=Payment

IC Value

ThVe IC Value of our journal is 62.78
Click Here

GLOBAL INDEXING



















Computer Science Directory. We are listed under Computer Research Institutes category

Share on Social media

Home | Privacy Policy | Terms & Conditions | Refund Policy | Feedback | Contact Us
Copyright © 2014 ijste.org All rights reserved