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ACTIVITY OF CRUDE EXTRACT CELLULASE PRODUCED BY Cellvibrio mixtus UV4 THAT ISOLATED FROM DADUK


Author(s):

Ummul Firmani , Faculty of Agriculture University of Muhammadiyah Gresik East Java, Indonesia; Ni’matuzahroh, Faculty of Science and Technology University of Airlangga Surabaya, East Java, Indonesia ; Sri Sumarsih, Faculty of Science and Technology University of Airlangga Surabaya, East Java, Indonesia

Keywords:

Activity of Cellulose, Cellulose, Cellvibrio Mixtus UV4, Daduk, Oreochromis, Niloticus

Abstract:

Daduk is a sugarcane leaf, waste of sugarcane plantation. Daduk containing a big number of cellulose that difficult to decompose naturally. Cellulose is a fiber that can be degrade by cellulase produced by organism especially microorganism, like bacteria or fungi. This research is to studi the cellulase activity produce by Cellvibrio mixtus UV4 that found in daduk. The procedure of this research are (1) Sterilization of tools and materials; (2) Rejuvenation isolates Cellvibrio mixtus UV4; (3) Production of cellulase enzymes with aerobic fermentation method using liquid CMC 1%; (4) Isolation of crude extract of cellulase enzymes by Somogiy-Nelson; (5) Determination of cellulase enzyme crude extract using UV-Vis spectrophotometer; and (6) Preparation of a standard curve of glucose by the method of Somogiy-Nelson. The cellulase activity is 0.026 U/mL until 0.039 U/mL during 8 days.


Other Details:

Manuscript Id :IJSTEV2I7003
Published in :Volume : 2, Issue : 7
Publication Date: 01/02/2016
Page(s): 245-248
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